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gerardo ferbeyre  (Addgene inc)


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    Structured Review

    Addgene inc gerardo ferbeyre
    Gerardo Ferbeyre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gerardo+ferbeyre/pLPC-Flag-PML-IV+(Plasmid+%2362804)/pm40404649-241-12-14
    Average 93 stars, based on 6 article reviews
    gerardo ferbeyre - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Retroviral:

    Article Title: Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature.
    Article Snippet: After selection, cells were sorted using EDTA, to ensure that the cells are in a single cell suspension, and filtering using 1/2–1/3 the diameter to sort them using BD FACSCanto II. .. – AoSMC-Flag/Flag-progerin: Retroviral particles containing pLPC-Flag and PLPC-Flag-progerin which were a gift from Gerardo Ferbeyre (Addgene plasmid # 69,059 and PLPC-Flag-progerin #69,061) were used to infect AoSMC, and the cells infected were selected with puromycin 4 μγ/ ml. .. – AoSMC inducible progerin-expressing cells: Lentiviral particles containing pLenti-CMVTRE3GNeo-GFP progerin, gift from Tom Misteli (Addgene plasmid # 118,710); pLenti-CMVTRE3G-GFP-laminA, gift from Tom Misteli (Addgene plasmid # 118,709); and the constitutive tetracycline repressor A3 mutant expressing pLenti-CMV-rtTA3, gift from Eric Campau (Addgene plasmid # 26,429) were used to infect AoSMC cells as described in [40].

    Plasmid Preparation:

    Article Title: Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature.
    Article Snippet: After selection, cells were sorted using EDTA, to ensure that the cells are in a single cell suspension, and filtering using 1/2–1/3 the diameter to sort them using BD FACSCanto II. .. – AoSMC-Flag/Flag-progerin: Retroviral particles containing pLPC-Flag and PLPC-Flag-progerin which were a gift from Gerardo Ferbeyre (Addgene plasmid # 69,059 and PLPC-Flag-progerin #69,061) were used to infect AoSMC, and the cells infected were selected with puromycin 4 μγ/ ml. .. – AoSMC inducible progerin-expressing cells: Lentiviral particles containing pLenti-CMVTRE3GNeo-GFP progerin, gift from Tom Misteli (Addgene plasmid # 118,710); pLenti-CMVTRE3G-GFP-laminA, gift from Tom Misteli (Addgene plasmid # 118,709); and the constitutive tetracycline repressor A3 mutant expressing pLenti-CMV-rtTA3, gift from Eric Campau (Addgene plasmid # 26,429) were used to infect AoSMC cells as described in [40].

    Article Title: Structural basis for the human SENP5's SUMO isoform discrimination.
    Article Snippet: .. FulllengthRGS-SENP5was a gift fromEdwardYeh (Addgeneplasmid#18053; http://n2t.net/addgene:18053; RRID:Addgene_18053), pLPC-Flag-PML-IV was a gift from Gerardo Ferbeyre (Addgene plasmid #62804; http://n2t. net/addgene:62804; RRID:Addgene_62804), pcDNA3-HA-SUMO1 was a gift from JunyingYuan (Addgeneplasmid#21154;http://n2t.net/addgene: 21154; RRID:Addgene_21154) and pcDNA3HA-SUMO2WTwas a gift from Guy Salvesen (Addgene plasmid #48967; http://n2t.net/addgene:48967; RRID:Addgene_48967)38,72–74. ..

    Article Title: Structural basis for the human SENP5’s SUMO isoform discrimination
    Article Snippet: .. Full-length RGS-SENP5 was a gift from Edward Yeh (Addgene plasmid #18053; http://n2t.net/addgene:18053 ; RRID:Addgene_18053), pLPC-Flag-PML-IV was a gift from Gerardo Ferbeyre (Addgene plasmid #62804; http://n2t.net/addgene:62804 ; RRID:Addgene_62804), pcDNA3-HA-SUMO1 was a gift from Junying Yuan (Addgene plasmid #21154; http://n2t.net/addgene:21154 ; RRID:Addgene_21154) and pcDNA3 HA-SUMO2 WT was a gift from Guy Salvesen (Addgene plasmid #48967; http://n2t.net/addgene:48967 ; RRID:Addgene_48967) , – . ..

    Infection:

    Article Title: Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature.
    Article Snippet: After selection, cells were sorted using EDTA, to ensure that the cells are in a single cell suspension, and filtering using 1/2–1/3 the diameter to sort them using BD FACSCanto II. .. – AoSMC-Flag/Flag-progerin: Retroviral particles containing pLPC-Flag and PLPC-Flag-progerin which were a gift from Gerardo Ferbeyre (Addgene plasmid # 69,059 and PLPC-Flag-progerin #69,061) were used to infect AoSMC, and the cells infected were selected with puromycin 4 μγ/ ml. .. – AoSMC inducible progerin-expressing cells: Lentiviral particles containing pLenti-CMVTRE3GNeo-GFP progerin, gift from Tom Misteli (Addgene plasmid # 118,710); pLenti-CMVTRE3G-GFP-laminA, gift from Tom Misteli (Addgene plasmid # 118,709); and the constitutive tetracycline repressor A3 mutant expressing pLenti-CMV-rtTA3, gift from Eric Campau (Addgene plasmid # 26,429) were used to infect AoSMC cells as described in [40].



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    93
    Addgene inc gerardo ferbeyre
    Gerardo Ferbeyre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gerardo+ferbeyre/pLPC-Flag-PML-IV+(Plasmid+%2362804)/pm40404649-241-12-14
    Average 93 stars, based on 1 article reviews
    gerardo ferbeyre - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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